0704-883-0675     |      dataprojectng@gmail.com

MOLECULAR CHARACTERIZATION OF MULTIDRUG RESISTANT BACTERIAL UROPATHOGENS FROM PREGNANT WOMEN IN IBADAN METROPOLIS, SOUTH-WEST NIGERIA

  • Project Research
  • 1-5 Chapters
  • Abstract : Available
  • Table of Content: Available
  • Reference Style: APA
  • Recommended for : Student Researchers
  • NGN 5000

ABSTRACT

Urinary tract infection (UTI) is one of the commonest infectious diseases of women worldwide, and pregnant women are more predisposed. This study aimed to determine the prevalence of bacterial infecting agents in UTI during pregnancy, to establish their antimicrobial susceptibility profiles, identify the socio-economic factors that promote UTI. Virulence factor genes (VFGs) of the multi-drug resistant bacterial uropathogens were determined in order to establish their pathotypes. Clean catch mid-stream urine samples were collected, processed and analyzed from a total of 650 women on their first visit to antenatal clinics of three major health facilities in Ibadan metropolis. Demographic information was collected using structured questionnaires. Dip-stick leucocyte esterase test was performed and urinary isolates were identified using standard conventional biochemical tests and further confirmed to specie level with Microbact® identification kits (Oxoid). Modified Kirby-Bauer disc diffusion method was used to determine the antibiotic susceptibility profile and interpreted as specified by the Clinical Laboratory Standard Institute (CLSI).Genomic DNA extraction and amplification of specific virulence genes associated with UTI by polymerase chain reaction (PCR) was carried out. Extended spectrum beta-lactamase production by the most prevalent multi-drug resistant Gram negative isolate was determined phenotypically and genotypically using universal primers.Molecular phylogenetic analysis by maximum likelihood method (MLM) was carried out for some sequenced isolates, and an evolutionary analysis was conducted using MEGA 7® . Significant bacteriuria was observed in 246(37.8%) antenatal patients, with 149(60.6%) being asymptomatic. The major bacterial species recovered included Staphylococcus epidermidis 51(21.9%), Escherichia coli 48(19.5%), Staphylococcus aureus 27(10.9%), Streptococcusspp vii 8 17(6.0%), Providencia stuartii 12(4.8%), Proteus mirabilis 13(5.2%),Staphyloccocus saprophyticus 12(4.9%),Enterobacter agglomerans 8(3.2%). Others including Staphylococcus sppranged from 0.4% to 2.3%. The highest prevalence of bacteriuria with respect to gestational age, women‟s age group, occupational status and educational level were 53.2% (n=131) third trimester, 44.2% (n=101) 25-30 years, 35.3% (n=87) housewives, and women with no formal education 32.1% (n=79) respectively and were statistically significant at P<0.05. Majority (69.5%) of the isolates were multidrug resistant. The isolates were most resistant to Cotrimoxazole (66.6%), and most susceptible to Nitrofurantoin (11.9% resistance). Phenotypic ESBL production was seen in 35(72.9%) of E. coli isolates; the ESBL genes amplified were bla CTX-M 10(100%), blaTEM 10(100%), bla OXA 10(100%), and bla SHV 7(70%). Virulence genes amplified in the analyzed MDR ESBL E. coli isolates were fim H 10(100%), pap 10(100%), cnf 5(50%), upa Bc 10(100%), qep 5(50%), Int 1 10(100%), IS 256 8(80%), amp C 8(80%), aac 1(10%), tsp 10(100%), yjaA 9(90%), but chuA gene was absent. Virulent extraintestinal phylogenetic group B2 strains of uropathogenic E. coli (UPEC) 7(70.0%) were identified. In the analyzed MDR S. aureus isolates, using simplex PCR, the following were amplified: mec A 2(20%), amp C 10(100%), ica D 8(80%), ica A 5(50%), Int 1 10(100%), IS 256 7(70%), upa Bc 10(100%), while pvl, hly, aer, qep and upa Cc genes were absent. The nucleotide sequence of 16SrRNA gene of all the MDR isolates used in the molecular analysis in comparison with those from GenBank database revealed 100-99% identity.





Related Project Materials

THE EFFECTS OF IMMUNIZATION AS IT AFFECTS CHILDREN HEALTH

ABSTRACT

This study was carried out on the effects of immunization as it affects children health. A cross-sectional survey was conducted...

Read more
THE EFFECT OF BRANDING AND PACKAGING OF LOCALLY MADE COSMETIC PRODUCTS ON THE BUYING DECISION OF CUSTOMERS

EXCERPT FROM THE STUDY

Competition within this industry has forced local company stakeholders to invest in more attracti...

Read more
THE EFFECT OF HUMAN RESOURCE MANAGEMENT (HRM) PRACTICES ON ORGANIZATIONAL PERFORMANCE

ABSTRACT

A human resource practice is considered the most critical for organizational performance. However,...

Read more
Design and Implementation of an Online Course Registration and Payment System for Bayero University, Kano

Background of the Study

Course registration and payment processes are integral to university administration, yet many in...

Read more
STRATEGIES FOR REDUCING MALNUTRITION ON CHILDREN'S ZERO TO FIVE YEARS

ABSTRACT

The study examines the causes of malnutrition in children from zero to five years. This study was undertaken ma...

Read more
An Examination of Political Legitimacy in Local Governance: The Case of Ajao LGA, Katsina State

Chapter One: Introduction

1.1 Background of the Study

Political legitimacy refers to the recognition and acceptance of authorit...

Read more
ATTITUDE OF CONSUMERS TOWARDS PUBLIC UTILITIES

EXCERPT FROM THE STUDY

In this study, our focus was to examine the attitude of consumers towards public utilities  ...

Read more
ATTITUDE OF EDUCATIONAL ADMINISTRATORS TO ICT USAGE IN SECONDARY SCHOOL MANAGEMENT (A CASE STUDY OF WUSE COMPREHENSIVE COLLEGE, ABUJA)

Abstract

This research work was carried to assess the administrators to ict usage in secondary school management (a case...

Read more
INNOVATIONS IN ASSESSING ADAPTABILITY AND RESILIENCE IN VOCATIONAL SETTINGS

Abstract: Innovations in assessing adaptability and resilience in vocational settings are c...

Read more
Assessing the Effect of Tax Holidays on Job Creation in Malumfashi LGA

Background of the Study

Tax holidays, which provide businesses with temporary relief from paying taxes,...

Read more
Share this page with your friends




whatsapp